Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Kinetic parameters of TrxRs from A. fumigatus , S. cerevisiae , and E. coli , with endogenous Trx as the substrate a

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Techniques: Activity Assay, Mutagenesis

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Kinetic parameters of TrxRs from A. fumigatus , S. cerevisiae , and E. coli , with endogenous Trx as the substrate a

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing Crystal structure of Af TrxR.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing Crystal structure of Af TrxR.

Techniques: Activity Assay, Mutagenesis

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Kinetic parameters of TrxRs from A. fumigatus , S. cerevisiae , and E. coli , with endogenous Trx as the substrate a

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Techniques: Activity Assay, Mutagenesis

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Techniques: Activity Assay, Mutagenesis

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Structure, Mechanism, and Inhibition of Aspergillus fumigatus Thioredoxin Reductase

doi: 10.1128/AAC.02281-18

Figure Lengend Snippet: Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Article Snippet: The multiazole-resistant (G54R) A. fumigatus isolate showed increased EbSe susceptibility, with an MIC of 16 µg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Isolate or quality control combination Description MIC (μg/ml) Isolates A. fumigatus ATCC MYA-3626 CLSI reference strain 32 Clinical isolates 1 Sputum, pan-azole resistant (TR 34 /L98H cyp51A mutation) 32 2 Sputum, multiazole resistant (G54R cyp51A mutation) 16 3 Tracheal aspirate 32 4 Lung tissue 32 5 Lung tissue 64 A. flavus Sinus tissue 32 Quality control combinations Amphotericin B vs A. fumigatus MYA-3626 0.5 Anidulafungin vs A. fumigatus ATCC MYA-3626 ≤ 0.008 Amphotericin B vs Candida parapsilosis ATCC 22019 0.25 Anidulafungin vs Candida parapsilosis ATCC 22019 1 Open in a separate window Antifungal activity of EbSe against Aspergillus reference and clinical isolates as determined by broth microdilution testing

Techniques: Activity Assay, Mutagenesis